Yes! If you put fire ant bait out too early, you will kill all the workers and then new queens can invade the area. In most states, fire ant mating flights become less common after the end of May. If you apply a fire ant bait too early in the spring, it is likely that the area will be quickly reinvaded by new queens. Learn how quickly the particular fire ant bait works, and choose the appropriate bait for your needs. See also FAQ #824: "Which bait do I use?"