The following was presented at the 2010 American Bee Research Conference in Orlando, FL.
23. Parker R., M.M. Guarna, S. Pernal & L.J. Foster- APIS MELLIFERA PROTEOMICS OF INNATE RESISTANCE (APIS) - The APIS project focuses on two diseases affecting honey bees in North America, American foulbrood (AFB) and Varroa mites. Although treatments are available, increased resistance and the concern of chemical residues makes selective breeding of resistant bee stock the most desirable solution. Our goal is to develop tools for accelerating and strengthening the selection process. These tools will be based on protein markers that indicate resistance and can predict whether a honey bee stock will be more likely to resist or tolerate disease. In our first year, we sampled diverse stocks (Figure), tested behavioural and physiological field traits, and are using Mass Spectrometry-based proteomics to compare protein expression. Analysis of ca. 500 quantified proteins in each tissue showed significant protein differences that were related to the characteristics of the bees sampled. For the 2009-2010 experiments, we acquired additional queens and have implemented a mating design to study heritability of the field traits and the protein markers associated with them. We are also collaborating with Jeff Pettis, USDA, to investigate the economic impact of disease on the beekeeping industry. Our recent progress will be presented.
More presentations from this conference can be found at Proceedings of the American Bee Research Conference 2010