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High-throughput SNP Genotyping in Cucurbita Pepo for Map Construction and Quantitative Trait Loci Mapping

Last Updated: February 22, 2012

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Released February 22, 2012

Cucurbita pepo is a member of the Cucurbitaceae family, the second most important horticultural family in terms of economic importance after Solanaceae. The "summer squash" types, including Zucchini and Scallop, rank among the highest-valued vegetables worldwide. There are few genomic tools available for this species. Recently, the first Cucurbita transcriptome, along with a large collection of Single Nucleotide Polymorphisms (SNPs), have been generated using massive sequencing. A customized 384-SNP set was selected to generate an Illumina Golden Gate assay used to construct the first SNP-based genetic map of Cucurbita and to map quantitative trait loci (QTL).

--continued on BMC Genomics, http://www.biomedcentral.com/1471-2164/13/80/abstract

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